Isolation of two strains of Acanthamoeba castellanii from human tissue and their pathogenicity and isoenzyme profiles.
J Clin Microbiol
; 18(6): 1405-12, 1983 Dec.
Article
en En
| MEDLINE
| ID: mdl-6655045
ABSTRACT
Two strains of amoebae, one (CDC01801) from the lung tissue of a patient who died of granulomatous amoebic encephalitis and the other (CDC01791) from the debrided tissue of a mandibular autograft, were isolated and identified as Acanthamoeba castellanii based on the morphological and immunofluorescent staining characteristics of the trophozoites and cysts. Both strains of amoebae caused cytopathic effects in mammalian cell cultures and destroyed the cell sheet. However, only the CDC01801 strain, on intranasal instillation into mice, produced the disease manifested by ruffled fur and aimless wandering, followed by coma and death within 30 days. The CDC01801 strain also differed consistently from CDC01791 and another nonpathogenic A. castellanii strain (ATCC 30,011) in isoenzyme makeup, a dissimilarity which probably reflects its pathogenic potential.
Texto completo:
1
Colección:
01-internacional
Asunto principal:
Amebiasis
/
Amoeba
Tipo de estudio:
Etiology_studies
/
Prognostic_studies
Límite:
Adult
/
Animals
/
Female
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Humans
/
Male
Idioma:
En
Revista:
J Clin Microbiol
Año:
1983
Tipo del documento:
Article