Increased fibronectin-receptor expression in colon carcinoma-derived HT 29 cells decreases tumorigenicity in nude mice.
Gastroenterology
; 106(1): 19-27, 1994 Jan.
Article
en En
| MEDLINE
| ID: mdl-8276181
ABSTRACT
BACKGROUND/AIMS:
Following malignant transformation, epithelial cells of colorectal carcinomas, unlike normal colonic epithelial cells, no longer express the alpha 5 beta 1 fibronectin receptor. We hypothesized that the loss of alpha 5 beta 1 expression might facilitate the tumorigenicity of transformed colonic cells.METHODS:
To examine this hypothesis, we established subclones of the human colon adenocarcinoma cell line HT 29, which differ in their fibronectin receptor expression and tested their tumorigenicity in nude mice.RESULTS:
Our data indicate that the capacity to form tumors in nude mice after subcutaneous injection was significantly lower for alpha 5-positive than for alpha 5-negative cell clones. In addition, tumors from clones expressing no detectable levels of alpha 5 beta 1 grew rapidly, whereas tumors expressing elevated levels of fibronectin receptor grew slowly. Despite similar rates of adhesion to fibronectin for alpha 5-positive and alpha 5-negative cell clones in vitro, deposition of fibronectin in tumor-surrounding stroma was increased in tumors derived from alpha 5-positive cells.CONCLUSIONS:
Our results indicate that an increase of the alpha 5 beta 1-mediated interaction of malignant cells with the extracellular matrix may be responsible for decreased tumorigenicity of malignant transformed cells in colorectal carcinomas.
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Colección:
01-internacional
Asunto principal:
Adenocarcinoma
/
Receptores de Fibronectina
/
Neoplasias del Colon
/
Trasplante de Neoplasias
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Gastroenterology
Año:
1994
Tipo del documento:
Article
País de afiliación:
Alemania