Identification of human p53 mutations with differential effects on the bax and p21 promoters using functional assays in yeast.
Oncogene
; 16(10): 1369-72, 1998 Mar 12.
Article
en En
| MEDLINE
| ID: mdl-9546439
ABSTRACT
Recent studies have suggested that a rare class of p53 mutants found in tumours has a subtle transcriptional defect affecting bax induction but not p21 induction. We have therefore developed simple functional assays in yeast which can be used to identify these mutants. Analysis of 51 different mutations observed in human tumours showed that all mutants tested scored as mutant with the bax reporter strain but nine scored as wild-type with the p21 reporter strain. These results, which can be explained by the lower affinity of the p53 protein for the bax site, may suggest that p21 is not the key target of p53 mutations in tumours. Since p21 status has recently been shown to modulate the chemotherapeutic and radiotherapeutic sensitivities of cancerous cells, the functional assays described here may have important clinical implications.
Buscar en Google
Colección:
01-internacional
Asunto principal:
Genes p53
/
Proteína p53 Supresora de Tumor
/
Proteínas Proto-Oncogénicas
/
Regiones Promotoras Genéticas
/
Ciclinas
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Mutación
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Oncogene
Asunto de la revista:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Año:
1998
Tipo del documento:
Article
País de afiliación:
Francia