Astrocytic glutamate uptake and prion protein expression.
Glia
; 25(3): 282-92, 1999 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-9932874
ABSTRACT
Factors influencing glutamate uptake by astrocytes may indirectly influence neuronal survival. Elevated extracellular glutamate may be excitotoxic or may exacerbate neurodegeneration in various neurological diseases. By using a cell culture model, we have investigated the influence of astrocytic prion protein (PrPc) expression on glutamate uptake. Type 1 astrocytes expressing PrPc have a higher rate of Na+-dependent glutamate uptake than PrPc-deficient type 1 astrocytes. This difference is exacerbated when serum free media is used to culture the astrocytes. Further analysis suggested that a decrease in substrate affinity is responsible for the sensitivity of PrP-deficient astrocytic glutamate uptake to culture conditions. PrPc has been shown to bind copper. Greater sensitivity of cells to copper concentrations may be responsible for the decreased substrate affinity observed. PrPc-deficient cerebellar cells are more sensitive to glutamate toxicity in the presence of copper. These results show that glutamate uptake from astrocytes is dependent on PrPc expression which in turn may be related to copper metabolism.
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Colección:
01-internacional
Asunto principal:
Priones
/
Astrocitos
/
ATPasa Intercambiadora de Sodio-Potasio
/
Ácido Glutámico
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Glia
Asunto de la revista:
NEUROLOGIA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Reino Unido