Cytotoxicity of Calcium Hydroxide on Human Umbilical Cord Mesenchymal Stem Cells
Pesqui. bras. odontopediatria clín. integr
; 20: e0044, 2020. tab, graf
Artigo
em Inglês
| LILACS, BBO
| ID: biblio-1135549
Biblioteca responsável:
BR1264.1
ABSTRACT
Abstract Objective: To examine the cytotoxicity of calcium hydroxide on human umbilical cord mesenchymal stem cells (HUCMSC) to understand the characteristics for use in regenerative dentistry procedures especially regenerative endodontics. Material and Methods: HUCMSC was isolated, cultured, and confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSC was cultured and divided into two groups, the control group (cultured in minimum essential medium (MEM) alpha) and calcium hydroxide group (cultured in MEM alpha and calcium hydroxide). Methyl-thiazole-tetrazolium (MTT) assay was done on different concentrations of calcium hydroxide (0.39 to 25 µg/mL) and the cells were observed and counted. One-way ANOVA test was used with a significance level set at 5%. Results: Flow cytometric analysis confirmed positive of CD73, CD90, CD105, negative of CD45 and CD34. A significant difference was found between the concentration of 6.25 and 3.125 µg/mL (p=0.004). There was no significant difference among 6.25, 12.5 and 25 µg/mL concentrations. There was also no significant difference among 0.39, 0.78, 1.56, and 3.125 µg/mL concentrations. Conclusion: Even though calcium hydroxide is a medicament of choice in clinical endodontics, it decreases the viability of HUCMSC. The lower the concentration of calcium hydroxide, the higher the viability of HUCMSC.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Temas:
Geral
Base de dados:
LILACS
Assunto principal:
Hidróxido de Cálcio
/
Sobrevivência Celular
/
Pesquisa com Células-Tronco
/
Células-Tronco Mesenquimais
/
Endodontia Regenerativa
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Pesqui. bras. odontopediatria clín. integr
Assunto da revista:
Odontologia
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Indonésia
Instituição/País de afiliação:
Universitas Airlangga/ID
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