Effect of phenylmethylsulfonyl fluoride, a protease inhibitor, on enamel surface remineralization
Braz. j. oral sci
; 22: e230883, Jan.-Dec. 2023. ilus
Article
em En
| LILACS, BBO
| ID: biblio-1399769
Biblioteca responsável:
BR218.1
ABSTRACT
Phenylmethylsulfonyl fluoride (PMSF) is a protease inhibitor widely used in research, but fluoride is released during its action and this knowledge has been neglected in dental research. Aim:
to evaluate if fluoride released by salivary protease action on PMSF affects enamel remineralization and fluoride uptake.Methods:
Groups of 10 enamel slabs, with caries-like lesions and known surface hardness (SH), were subjected to one of the following treatment groups Stimulated human saliva (SHS), negative control; SHS containing 1.0 µg F/mL (NaF), positive control; and SHS containing 10, 50 or 100 µM PMSF. The slabs were subjected to a pH-cycling regimen consisting of 22 h/day in each treatment solution and 2 h/day in a demineralizing solution. After 12 days, SH was again measured to calculate the percentage of surface hardness recovery (%SHR), followed by enamel fluoride uptake determination. The time-related fluoride release from 100.0 µM PMSF by SHS action was also determined. Data were analyzed by ANOVA followed by Newman-Keuls test.Results:
The release of fluoride from PMSF by SHS was rapid, reaching a maximum value after 10 min. Fluoride released from PMSF was more effective in enhancing %SHR and increasing fluoride uptake in enamel compared with SHS alone (p < 0.05); furthermore, it was equivalent to the positive control (p > 0.05).Conclusion:
In conclusion, fluoride released by saliva from PMSF is available to react with enamel and needs to be taken into account in research using this protease inhibitorAssuntos
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Fluoreto de Fenilmetilsulfonil
/
Inibidores de Proteases
/
Remineralização Dentária
/
Esmalte Dentário
Idioma:
En
Revista:
Braz. j. oral sci
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Brasil
/
Estados Unidos