Effects of daidzein on rat ovary against ischemia-reperfusion
Acta cir. bras
; 38: e384423, 2023. tab, graf, ilus
Artigo
em Inglês
| LILACS, VETINDEX
| ID: biblio-1519872
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
Purpose: Our aim was to investigate protective effects of daidzein treatment on ischemia-reperfusion (I/R) injury-induced ovarian tissue by immunohistochemical techniques. Methods: Thirty Sprague Dawley female rats were categorized into three groups as sham, I/R group, and I/R+daidzein groups. Bloods were analyzed for malondialdehyde (MDA), glutathione peroxidase (GSH), and myeloperoxidase (MPO), and ovaries were processed for histological tissue protocol. Results: Both MDA and MPO values were increased in I/R group compared to sham and I/R+daidzein groups. GSH content was increased in I/R+daidzein group compared to I/R groups. In I/R group, theca and follicular cells were degenerated with apoptosis and dilatation and congestion, edema. In I/R+daidzein group, daidzein improved pathologies. In the I/R group, Bax expression was positive with follicular cells, granulosa cells and inflammatory cells. In the I/R+daidzein group, positive Bax reaction was observed in the epithelial, antral, and inflammatory cells. In I/R group, Bcl-2 reaction was in germinative epithelial cells, cells of antral follicle. In the I/R+daidzein group, Bcl-2 expression level was reduced after daidzein treatment. Conclusions: After the I/R procedure, ovarian cells and follicles were degenerated with apoptosis and inflammation. After daidzein treatment, Bax and Bcl-2 signal were decreased. It was observed that daidzein stopped the apoptotic process.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Ovário
/
Imuno-Histoquímica
/
Reperfusão
/
Isquemia
/
Isoflavonas
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2023
Tipo de documento:
Artigo
País de afiliação:
Turquia
Instituição/País de afiliação:
Dicle University/TR
/
Division of Gynecology and Obstetrics/TR
/
Division of Perinatology/TR
/
Diyarbakir Bower Hospital/TR
/
Eyyübiye Education and Research Hospital/TR
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