Characterization of a fluorometric method for lipoprotein lipase
Arch. med. res
; 25(3): 331-5, 1994. tab, ilus
Article
em En
| LILACS
| ID: lil-198816
Biblioteca responsável:
MX1.1
RESUMO
A fluorometric assay for determining lipoprotein lipase (LpL) activity is described. Dibutyrilfluorescine (DBF) was used as substrate for the enzyme and the fluorescine liberated by enzymatic hydrolysis of the substrate was measured. Extracts of acetone powder from adipose tissue as an enzyme source showed characteristics of lipoprotein lipase activity, i.e., inhibition by NaCl and optimum activity in alkaline pH. There was close agreement in LPL activity when the same sample was measured simultaneously using either dibutyrilfluorescine or tri[9, 10 3H]oleylglycerol as substrate. The extent of inhibition of lipoprotein lipase by NaCl was similar with both methods. The fluorometric method detected changes in LPL activity in heart and adipose tissue realted to the nutritional status of the animal with the same specificity and sensitivity than did the radioactive method. The flluorometric method is as sensitive, less expensive and less time consuming than the radioactive method:
Buscar no Google
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Tecido Adiposo
/
Estado Nutricional
/
Receptores de Lipoproteínas
/
Fluorometria
/
Lipase Lipoproteica
Limite:
Animals
Idioma:
En
Revista:
Arch. med. res
Assunto da revista:
MEDICINA
Ano de publicação:
1994
Tipo de documento:
Article