Vimentin expression and the influence of Matrigel in cell lines of head and neck squamous cell carcinoma
Braz. oral res
; 25(3): 235-240, May-June 2011. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-590041
Biblioteca responsável:
BR1.1
ABSTRACT
Vimentin is a cytoeskeletal intermediate filament protein commonly observed in mesenchymal cells; however, it can also be found in malignant epithelial cells. It is demonstrated in several carcinomas, such as those of the cervix, breast and bladder, in which it is widely used as a marker of the epithelial to mesenchymal transition that takes place during embryogenesis and metastasis. Vimentin is associated with tumors that show a high degree of invasiveness, being detected in invasion front cells. Its expression seems to be influenced by the tumor microenvironment. The aim of this study was to evaluate vimentin expression in head and neck squamous cell carcinoma (HNSCC) cell lines, and to investigate the contribution of the microenvironment to its expression. HNSCC cell lines (HN6, HN30 and HN31) and an immortalized nontumorigenic cell line (HaCaT) were submitted to a three-dimensional assay with Matrigel. Cytoplasmatic staining of the HN6 cell line cultured without Matrigel and of the HN30 and HN31 cell lines cultured with Matrigel was demonstrated through immunohistochemistry. Western Blotting revealed a significant decrease in vimentin expression for the HN6 cell line and a significant increase for the HN30 and HN31 cell lines cultured with Matrigel. The results suggest that vimentin can be expressed in HNSCC cells and its presence is influenced by the microenvironment of a tumor.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Temas:
Geral
/
Tipos de Câncer
/
Outros Tipos
Base de dados:
LILACS
Assunto principal:
Proteoglicanas
/
Vimentina
/
Carcinoma de Células Escamosas
/
Colágeno
/
Laminina
/
Neoplasias de Cabeça e Pescoço
/
Proteínas de Neoplasias
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. oral res
Assunto da revista:
Odontologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
University of São Paulo/BR
Similares
MEDLINE
...
LILACS
LIS