[Ameliorative effects of adrenomedullin on rat cardiac sarcoplasmic reticulum injured by isoproterenol].
Sheng Li Xue Bao
; 53(5): 364-8, 2001 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-11833420
ABSTRACT
Five indices were used to assess the degree of the ameliorative effects of adrenomedullin (Adm) in isoproterenol (ISO) induced injury of cardiac sarcoplasmic reticulum (SR) of rats +/- LV dp/dtmax, SR Ca2+ uptake, Ca2+ release (in nmol/mg SR protein) and Ca(2+)-ATPase activity. Adm (5 x 10(-5) mol/L) was perfused into rat heart before cardiac function +/- LV dp/dtmax was determined. Cardiac SR was purified and SR Ca2+ uptake, Ca2+ release, Ca(2+)-ATPase activity, and 3H-ryanodine maximal binding (Bmax) were measured. After perfusion of Adm (5 x 10(-5) mol/L), the +LV dp/dtmax and -LV dp/dtmax in ISO injured heart were increased by 16.9% (2,135 +/- 281 vs 1980 +/- 302, P < 0.05) and 29.2% (1375 +/- 267 vs 1064 +/- 355, P < 0.05), respectively. Cardiac SR Ca2+ uptake and Ca2+ release were increased by 23.0% (15.0 +/- 1.4 vs 12.2 +/- 1.2, P < 0.01) and 43.5% (6.6 +/- 1.0 vs 4.6 +/- 0.6, P < 0.01), respectively. Ca(2+)-ATPase activity and 3H-ryanodine binding were increased by 23.0% (P < 0.01) and 42.2% (P < 0.05), respectively. It seems that Adm exerted a protective effect on cardiac function in injured rat heart by enhancing SR Ca2+ uptake and release, suggesting that Adm may play a therapeutic role in myocardial injury.
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Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Retículo Sarcoplasmático
/
Traumatismo por Reperfusão Miocárdica
/
Cálcio
Limite:
Animals
Idioma:
Zh
Revista:
Sheng Li Xue Bao
Ano de publicação:
2001
Tipo de documento:
Article