Response to ADP-ribose by activation of TRPM2 in the CRI-G1 insulinoma cell line.
J Membr Biol
; 191(3): 201-7, 2003 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-12571754
ABSTRACT
The response to intracellular ADP-ribose in the rat CRI-G1 insulinoma cell line was studied using a patch-clamp method. Dialysis of ADP-ribose into cells induced a response in a dose-dependent manner. The reversal potentials in various solutions showed that the ADP-ribose-gated channel was a Ca2+-permeable nonselective cation channel. In inside-out recordings, ADP-ribose and b-NAD induced responses in the same patch. The single-channel current-voltage relationships for ADP-ribose- and b-NAD-induced responses were almost identical, indicating that ADP-ribose and b-NAD activated the same channel. The physiological properties of the ADP-ribose-gated channel are similar to those we reported previously for the cloned transient receptor potential channel TRPM2. Moreover, RT-PCR analysis showed that TRPM2 was abundantly expressed in CRI-G1 cells, suggesting that the ADP-ribose-gated channel represents the native TRPM2 channel in CRI-G1 cells. These results suggest that ADP-ribose can be an endogenous modulator of Ca2+ influx through the TRPM2 channel into CRI-G1 cells.
Buscar no Google
Coleções:
01-internacional
Temas:
Geral
/
Tipos_de_cancer
/
Outros_tipos
Base de dados:
MEDLINE
Assunto principal:
Canais de Cálcio
/
Adenosina Difosfato Ribose
/
Insulinoma
/
Canais Iônicos
/
Proteínas de Membrana
Idioma:
En
Revista:
J Membr Biol
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Japão