Expression of the acetylcholine release mechanism in various cells and reconstruction of the release mechanism in non-releasing cells.
Life Sci
; 72(18-19): 2029-38, 2003 Mar 28.
Article
em En
| MEDLINE
| ID: mdl-12628453
ABSTRACT
After loading cells in culture with acetylcholine (ACh), it was possible to identify cells that express a calcium-dependent release mechanism and cells that do not release. Mediatophore transfection restored the release capability of non-releasing cells. The transfection of choline acetyltransferase and the vesicular ACh transporter (VAChT) in cells that have already mediatophore in their membrane enables to study the effect of VAChT on the release kinetics. We also studied the properties of the mediatophore "pore" as a function of the concentration of ACh and also its temporal properties. A reconstruction of the release mechanism in cells particularly graftable cells, appears now possibly for ACh and probably for other transmitters.
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Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Acetilcolina
/
Proteínas de Transporte
/
Proteínas de Transporte Vesicular
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Life Sci
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
França