Activities of HIV-RNA targeted ribozymes transcribed from a 'shot-gun' type ribozyme-trimming plasmid.
Nucleic Acids Symp Ser
; (27): 15-6, 1992.
Article
em En
| MEDLINE
| ID: mdl-1289797
ABSTRACT
We have constructed a "shot-gun" type ribozyme-trimming system. By concatenating several units, each consisting of a trans-acting ribozyme (targeted to HIV-RNA) and cis-acting ribozymes (trimming 5'- and 3'-ends of the trans-acting ribozyme), several kinds of trans-acting ribozymes can be liberated upon transcription and self-cleavage. Since each liberated HIV-RNA-targeted ribozymes can work independently, they can simultaneously cleave HIV-RNA at several different sites. Ribozymes were targeted at relatively conserved GUC-containing sites at LTR, gag and tat regions.
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Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Transcrição Gênica
/
RNA Viral
/
RNA Catalítico
/
HIV
Idioma:
En
Revista:
Nucleic Acids Symp Ser
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Japão