The protective effect of fermented milk kefir on radiation-induced apoptosis in colonic crypt cells of rats.
J Radiat Res
; 44(2): 111-5, 2003 Jun.
Article
em En
| MEDLINE
| ID: mdl-13678339
ABSTRACT
To evaluate the effect of fermented milk kefir on X-ray-induced apoptosis in the colon of rats, we examined the apoptotic index, the mean number of apoptotic cells detected by H&E staining per crypt in the colon, in control rats and kefir-pretreated rats drinking kefir for 12 days before irradiation. Apoptotic cells were confirmed by TUNEL staining, and active caspase-3 expression was studied by immunohistochemistry. The cell position of apoptotic cells and active caspase-3 positive cells were examined. The apoptotic index of kefir-treated rats was significantly (p < 0.05) decreased 2 h after 1 Gy irradiation in comparison with control rats at crypt cell positions 1-3, 5-7, 13, and 15. Active caspase-3 expression in the kefir-treated rats was also significantly (p < 0.05) reduced in comparison with control rats 2 h after 1 Gy irradiation at crypt cell positions 1-4, 13, and 15. This study indicated that kefir protects colonic crypt cells against radiation-induced apoptosis, which was most pronounced in the stem cell region of the crypt. The antiapoptotic effect of fermented milk kefir was due to the inhibition of caspase-3 activation.
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Coleções:
01-internacional
Temas:
Agentes_cancerigenos
Base de dados:
MEDLINE
Assunto principal:
Lesões por Radiação
/
Proteção Radiológica
/
Apoptose
/
Colo
/
Leite
Limite:
Animals
Idioma:
En
Revista:
J Radiat Res
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Japão