[An automatic gas scanning device, technical improvement for obtaining a favourable atmosphere for in vitro culture of anaerobic bacteria]. / Balayage gazeux à l'aide d'un automate, une amélioration technique d'obtention d'une atmosphère propice à la culture en jarre des bactéries anaérobies.
Ann Biol Clin (Paris)
; 50(4): 239-45, 1992.
Article
em Fr
| MEDLINE
| ID: mdl-1456502
ABSTRACT
All methods for growth of anaerobic bacteria on solid media depend on the elimination of atmospheric O2 through use of a palladium catalyst (Deoxo-Catalyst), active in presence of at least 5% H2 with resultant formation of water. Anaerobic chambers and jars are the two conventional methods employed. Both are based on the elimination of air by means of a pump and its replacement with gas from a cylinder (evacuation-replacement technique). An alternative chemical technique for use in anaerobic jars consists of adding internal gas-generating sachets. The former techniques are more efficient but the trend, particularly in the clinical laboratories, is to use the simpler chemical system that has two inconveniences a slow establishment of anaerobiosis, and a high cost. We propose a new system that does not require a vacuum pump and consists in flushing anaerobic jars with a convenient gas mixture (H2, CO2, N2 4.5; 5; 90.5 v/v) by means of an automaton regulating both time and gas flow. Gas-liquid chromatography analysis of the gas inside the jar shows a rapid elimination of gaseous O2, whose residual concentration is low enough to permit growth of all anaerobes of clinical interest, including those which are more O2-sensitive. Comparative qualitative and quantitative data obtained with all available techniques demonstrate the advantages of the new system.
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Coleções:
01-internacional
Temas:
Agentes_cancerigenos
Base de dados:
MEDLINE
Assunto principal:
Bactérias Anaeróbias
/
Técnicas Bacteriológicas
Tipo de estudo:
Qualitative_research
Idioma:
Fr
Revista:
Ann Biol Clin (Paris)
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
França