The utility of DNA microarrays for characterizing genotoxicity.
Environ Health Perspect
; 112(4): 420-2, 2004 Mar.
Article
em En
| MEDLINE
| ID: mdl-15033590
ABSTRACT
Microarrays provide an unprecedented opportunity for comprehensive concurrent analysis of thousands of genes. The global analysis of the response of genes to a toxic insult (toxicogenomics), as opposed to the historical method of examining a few select genes, provides a more complete picture of toxicologically significant events. Here we examine the utility of microarrays for providing mechanistic insights into the response of cells to DNA damage. Our data indicate that the value of the technology is in its potential to provide mechanistic insight into the mode of action of a genotoxic compound. Array-based expression profiling may be useful for differentiating compounds that interact directly with DNA from those compounds that are genotoxic via a secondary mechanism. As such, genomic microarrays may serve as a valuable alternative methodology that helps discriminate between these two classes of compounds. Key words biomarkers, gene expression profile, genetic toxicology, mechanism of action, toxicogenomics.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Dano ao DNA
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
Toxicogenética
/
Poluentes Ambientais
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Environ Health Perspect
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos