Assessment of determinants affecting the dual topology of hepadnaviral large envelope proteins.
J Gen Virol
; 85(Pt 5): 1221-1225, 2004 May.
Article
em En
| MEDLINE
| ID: mdl-15105538
ABSTRACT
For functional diversity, the large (L) envelope protein of hepatitis B virus (HBV) acquires a dual transmembrane topology via co-translational membrane integration of the S region and partial post-translational translocation of the preS subdomain. Because each process requires the second transmembrane segment (TM2), we explored the action of this determinant by using protease protection analysis of mutant L proteins. We demonstrated that neither the disruption of a leucine zipper-like motif by multiple alanine substitutions nor the flanking charges of TM2 affected the topological reorientation of L. The dispensability of both putative subunit interaction modules argues against a link between preS post-translocation and envelope assembly. Phenotypic mixing experiments revealed that the preS and S protein domains of the related duck HBV L polypeptide failed to substitute functionally for the topogenic elements of HBV in directing the correct L topogenesis, implicating different translocation mechanisms used by the two hepadnavirus genera.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Processamento de Proteína Pós-Traducional
/
Proteínas do Envelope Viral
/
Vírus da Hepatite B
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Alemanha