[Enhancement effect of IL-2 on the proliferation of cultured mammary carcinoma cell line Bcap-37].

ABSTRACT

AIM: To investigate the effects of IL-2 on the proliferation of human mammary carcinoma cell line Bcap-37 and explore its possible mechanism. METHODS: Enhancement effect of IL-2 on proliferation of cultured Bcap-37 cells, the IL-2 expression on the cells, the expressions of IL-2Ralpha, beta, gamma mRNAs in the cells, the effect of IL-2 on DNA content at various periods of cell cycle and on Ca(2+) concentration in the cells were detected or observed by MTT colorimetry, immunohistochemical staining, RT-PCR, flow cytometry and laser scanning confocal microscope, respectively. RESULTS: IL-2 of 1x10(5) to 1x10(6) U/L significantly stimulated the proliferation of cultured Bcap-37 cells. IL-2 was secreted and IL-2Ralpha, beta and gamma were expressed by cultured Bcap-37 cells. IL-2(1x10(5) U/L) increased ratio of Bcap-37 cells in G(2) and S phases and decreased the Ca(2+) release from Bcap-37 cells. CONCLUSION: IL-2 significantly enhance the proliferation of mammary carcinoma cell line Bcap-37. The enhancement effect of IL-2 on Bcap-37 cell proliferation is possibly related to the expression of IL-2R and decreased Ca(2+) concentration in the cells.