Molecular recognition by a binary code.
J Mol Biol
; 348(5): 1153-62, 2005 May 20.
Article
em En
| MEDLINE
| ID: mdl-15854651
ABSTRACT
Functional antibodies were obtained from a library of antigen-binding sites generated by a binary code restricted to tyrosine and serine. An antibody raised against human vascular endothelial growth factor recognized the antigen with high affinity (K(D)=60 nM) and high specificity in cell-based assays. The crystal structure of another antigen binding fragment in complex with its antigen (human death receptor DR5) revealed the structural basis for this minimalist mode of molecular recognition. Natural antigen-binding sites are enriched for tyrosine and serine, and we show that these amino acid residues are intrinsically well suited for molecular recognition. Furthermore, these results demonstrate that molecular recognition can evolve from even the simplest chemical diversity.
Buscar no Google
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Serina
/
Tirosina
/
Sítios de Ligação de Anticorpos
/
Fragmentos Fab das Imunoglobulinas
/
Receptores do Fator de Necrose Tumoral
/
Fator A de Crescimento do Endotélio Vascular
/
Especificidade de Anticorpos
Limite:
Humans
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos