Cadmium-induced plant stress investigated by scanning electrochemical microscopy.
J Exp Bot
; 56(421): 2831-8, 2005 Nov.
Article
em En
| MEDLINE
| ID: mdl-16216848
ABSTRACT
In vivo oxygen evolution above single stomata in Brassica juncea has been used to investigate, for the first time, the effect of Cd-induced stress as imaged by scanning electrochemical microscopy (SECM). SECM images showed a clear stomatal structure-a pore, whose aperture is modulated by two guard cells, serving as the conduit for the oxygen produced. Lower stomatal density and larger stoma size were found in plants treated with 0.2 mM CdCl2 compared with control plants. Either the introduction of Cd caused a slower cell replication in the plane of the epidermis, hence fewer stomata, and/or the number of open stomata was reduced when plants were under Cd-stress. Oxygen evolution above individual stomatal complexes in Cd-treated plants was lower than that from control plants, as determined from the electrochemical current above the middle of each stoma. All guard cells under illumination were swollen, indicating that the stomata were open in both control and treated plants. Thus, decreased oxygen evolution in response to Cd cannot be attributed to simple closing of the stomata, but to a lower photosynthetic yield. SECM provides an excellent tool for monitoring the effects of Cd on photosynthetic activity at the scale of individual stomata.
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Coleções:
01-internacional
Temas:
Agentes_cancerigenos
Base de dados:
MEDLINE
Assunto principal:
Cádmio
/
Microscopia Eletrônica de Varredura
/
Eletroquímica
/
Mostardeira
Idioma:
En
Revista:
J Exp Bot
Assunto da revista:
BOTANICA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Canadá