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Comparative study of ortho- and meta-nitrated inhibitors of catechol-O-methyltransferase: interactions with the active site and regioselectivity of O-methylation.
Palma, P N; Rodrigues, M L; Archer, M; Bonifácio, M J; Loureiro, A I; Learmonth, D A; Carrondo, M A; Soares-da-Silva, P.
Afiliação
  • Palma PN; Department of Research and Development, Bial., A. Av. da Siderurgia Nacional, 4745-457 S. Mamede do Coronado, Trofa, Portugal. nuno.palma@bial.com
Mol Pharmacol ; 70(1): 143-53, 2006 Jul.
Article em En | MEDLINE | ID: mdl-16618795
ABSTRACT
In this work, we present a comparative case study of "ortho-" and "meta-nitrated" catecholic inhibitors of catechol-O-methyltransferase (COMT), with regard to their interaction with the catalytic site of the enzyme and the in vitro regioselective formation of their mono-O-methyl ether metabolites. In particular, the effects of altering the attachment position of the inhibitors' side-chain substituent, within the classic nitrocatechol pharmacophore, were investigated. For this purpose, we compared two simple regioisomeric nitrocatechol-type inhibitors of COMT, BIA 3-228 and BIA 8-176, which contain the benzoyl substituent attached at the meta and ortho positions, respectively, relative to the nitro group. The two compounds were slowly O-methylated by COMT in vitro, but the particular substitution pattern of each compound was shown to have a profound impact on the regioselectivity of their O-methylation. To provide a plausible interpretation of these results, a comprehensive analysis of the protein-inhibitor interactions and of the relative chemical susceptibility to O-methylation of the catechol hydroxyl groups was performed by means of docking simulations and ab initio molecular orbital calculations. The major structural and chemical factors that determine the enzyme regioselectivity of O-methylation were identified, and the X-ray structure of the complex of COMT with S-adenosyl-l-methionine and BIA 8-176 is herein disclosed. This is the first reported structure of the soluble form of COMT complexed with a nitrocatecholic inhibitor having a bulky substituent group in adjacent position (ortho) to the nitro group. Structural and dynamic aspects of this complex are analyzed and discussed, in the context of the present study.
Assuntos
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Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Benzofenonas / Inibidores Enzimáticos / Inibidores de Catecol O-Metiltransferase / Nitrofenóis Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Mol Pharmacol Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Portugal
Buscar no Google
Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Benzofenonas / Inibidores Enzimáticos / Inibidores de Catecol O-Metiltransferase / Nitrofenóis Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Mol Pharmacol Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Portugal