Phospholipase D1 is associated with amyloid precursor protein in Alzheimer's disease.
Neurobiol Aging
; 28(7): 1015-27, 2007 Jul.
Article
em En
| MEDLINE
| ID: mdl-16797788
ABSTRACT
Amyloid precursor protein (APP) is a widely expressed transmembrane protein of unknown function that is involved in the pathogenesis of Alzheimer's disease (AD). We investigated the involvement of phospholipase D (PLD) in the pathophysiology of AD. We showed dramatic upregulation of PLD1 immunoreactivity in reactive astroglial cells in brain tissue sections from authentic AD patients. Expression and activity of PLD1 were up-regulated in brain tissues from AD patients, especially caveolae membrane fraction, compared with those of control brains. Interestingly, PLD1 physically interacts and colocalizes with APP and caveolin-3. We found that APP was associated with the pleckstrin homology domain of PLD1, and the amyloid region of APP interacted with PLD. Elevated expression of APP stimulated PLD activity in human astroglioma cells. These results suggest that up-regulation of PLD might have a role in the neuronal pathology associated with AD.
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Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Fosfolipase D
/
Encéfalo
/
Precursor de Proteína beta-Amiloide
/
Doença de Alzheimer
Tipo de estudo:
Risk_factors_studies
Limite:
Aged
/
Animals
/
Female
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Humans
Idioma:
En
Revista:
Neurobiol Aging
Ano de publicação:
2007
Tipo de documento:
Article