Optimization of the delivery of heterologous proteins by the Salmonella enterica serovar Typhimurium type III secretion system for vaccine development.
Infect Immun
; 74(10): 5826-33, 2006 Oct.
Article
em En
| MEDLINE
| ID: mdl-16988261
ABSTRACT
Type III protein secretion systems, which are organelles with the capacity to deliver bacterial proteins into host cells, have been adapted to deliver heterologous antigens for vaccine development. A limitation of these antigen delivery systems is that some proteins are not amenable to secretion through this pathway. We show here that proteins from the simian and human immunodeficiency viruses that are not permissive for secretion through a Salmonella enterica serovar Typhimurium type III secretion system can be modified to travel this secretion pathway by introduction of discrete mutations. Proteins optimized for secretion were presented more efficiently via the major histocompatibility complex class I pathway and were able to induce a better immune response.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Salmonella typhimurium
/
Produtos do Gene gag
/
Proteínas dos Retroviridae
/
HIV
/
Vírus da Imunodeficiência Símia
/
Vacinas contra a AIDS
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Infect Immun
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos