Computational design of peptides that target transmembrane helices.
Science
; 315(5820): 1817-22, 2007 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-17395823
ABSTRACT
A variety of methods exist for the design or selection of antibodies and other proteins that recognize the water-soluble regions of proteins; however, companion methods for targeting transmembrane (TM) regions are not available. Here, we describe a method for the computational design of peptides that target TM helices in a sequence-specific manner. To illustrate the method, peptides were designed that specifically recognize the TM helices of two closely related integrins (alphaIIbbeta3 and alphavbeta3) in micelles, bacterial membranes, and mammalian cells. These data show that sequence-specific recognition of helices in TM proteins can be achieved through optimization of the geometric complementarity of the target-host complex.
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Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Engenharia de Proteínas
/
Membrana Celular
/
Complexo Glicoproteico GPIIb-IIIa de Plaquetas
/
Integrina alfaVbeta3
Limite:
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos