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The overexpression of polycomb group proteins Bmi1 and EZH2 is associated with the progression and aggressive biological behavior of hepatocellular carcinoma.
Sasaki, Motoko; Ikeda, Hiroko; Itatsu, Keita; Yamaguchi, Junpei; Sawada, Seiko; Minato, Hiroshi; Ohta, Tetsuo; Nakanuma, Yasuni.
Afiliação
  • Sasaki M; Department of Human Pathology, Kanazawa University Graduate School of Medicine, Kanazawa, Japan.
Lab Invest ; 88(8): 873-82, 2008 Aug.
Article em En | MEDLINE | ID: mdl-18591938
ABSTRACT
Polycomb-group proteins Bmi1 and EZH2 are involved in the malignant transformation and biological aggressiveness of several human carcinomas. We herein examined the significance of the Bmi1 and EZH2 expression in hepatocellular carcinoma (HCC) and its preneoplastic lesions, dysplastic nodules. The expression of Bmi1 and EZH2 were examined immunohistochemically in HCC (n=27) and dysplastic nodules (n=14), and combined hepatocellular and cholangiocarcinoma (HC-CC) (n=14). The effect of Bmi1 and EZH2 knockdown was examined in cultured HCC cells (HuH7 and HepG2) using siRNA. It was determined that Bmi1 was constantly expressed in cholangiocytes, but not in hepatocytes, and EZH2 was detected in neither cholangiocytes nor hepatocytes. Bmi1 and EZH2 were overexpressed in HCC and more extensively in HC-CC (P<0.01). Interestingly, Bmi1 and EZH2 were not overexpressed in the dysplastic nodules. The expression of Bmi1 and EZH2 was heterogeneous and associated with vascular infiltration, the histological grades, and the cell proliferation activity in HCC and HC-CC. In cultured carcinoma cells overexpressing Bmi1 and EZH2, knockdown of Bmi1 and EZH2 resulted in decreased cell proliferation activities. Therefore, the overexpression of polycomb-group proteins Bmi1 and EZH2 is associated with the malignant progression of HCC, thereby reflecting the aggressive biological behavior in HCC and HC-CC.
Assuntos

Texto completo: 1 Coleções: 01-internacional Temas: Geral / Tipos_de_cancer / Outros_tipos Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Fatores de Transcrição / Proteínas Nucleares / Transformação Celular Neoplásica / Proteínas Proto-Oncogênicas / Carcinoma Hepatocelular / Proteínas de Ligação a DNA / Neoplasias Hepáticas Tipo de estudo: Risk_factors_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Lab Invest Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Temas: Geral / Tipos_de_cancer / Outros_tipos Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Fatores de Transcrição / Proteínas Nucleares / Transformação Celular Neoplásica / Proteínas Proto-Oncogênicas / Carcinoma Hepatocelular / Proteínas de Ligação a DNA / Neoplasias Hepáticas Tipo de estudo: Risk_factors_studies Limite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Lab Invest Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Japão