[Promoter activity of B-cell maturation antigen gene in mice].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 24(8): 778-80, 2008 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-18687216
ABSTRACT
AIM:
To investigate the promoter activity of BCMA gene 5'-flanking regionfor the study the regulation mechanism of BCMA expression.METHODS:
Luciferase reporter plasmids containing 5'-flanking region of BCMA gene and serial deletions of the fragment were constructed. The luciferase expression was observed after these reporters were transfected into J558L, 293T and Hela cells.RESULTS:
The highest transcriptional activation was expressed in pGL3-B157 reporter, followed by pGL3-B607, pGL3-B359, pGL3-B93, and pGL3-B820 in sequence. The highest transcriptional activation was in J558L cell.CONCLUSION:
The 5'-flanking sequence from -820 to +145 bp of BCMA is of promoter activity. Serial deletion analysis of the promoter region of BCMA gene suggests the sequence from -93 to +145 could be a core promoter region.
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Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Regiões Promotoras Genéticas
/
Antígeno de Maturação de Linfócitos B
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
China