Plant proteins involved in Agrobacterium-mediated genetic transformation.
Annu Rev Phytopathol
; 48: 45-68, 2010.
Article
em En
| MEDLINE
| ID: mdl-20337518
ABSTRACT
Agrobacterium species genetically transform plants by transferring a region of plasmid DNA, T-DNA, into host plant cells. The bacteria also transfer several virulence effector proteins. T-DNA and virulence proteins presumably form T-complexes within the plant cell. Super-T-complexes likely also form by interaction of plant-encoded proteins with T-complexes. These protein-nucleic acid complexes traffic through the plant cytoplasm, enter the nucleus, and eventually deliver T-DNA to plant chromatin. Integration of T-DNA into the plant genome establishes a permanent transformation event, permitting stable expression of T-DNA-encoded transgenes. The transformation process is complex and requires participation of numerous plant proteins. This review discusses our current knowledge of plant proteins that contribute to Agrobacterium-mediated transformation, the roles these proteins play in the transformation process, and the modern technologies that have been employed to elucidate the cell biology of transformation.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Plantas
/
Rhizobium
/
Transformação Bacteriana
/
Plasmídeos Indutores de Tumores em Plantas
Idioma:
En
Revista:
Annu Rev Phytopathol
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos