Simultaneous evaluation of mRNAs of dopamine ?-hydroxylase, tyrosine hydroxylase and proenkephalin a from three human pheochromocytomas.

Using the rabbit reticulocyte cell-free translation system, the relative proportions of in vitro translatable mRNAs of three proteins in three human pheochromocytomas: tyrosine hydroxylase (TH), dopamine ?-hydroxylase (DBH) and proenkephalin A have been compared. TH expression appeared rather constant in the three tumors. In contrast, those of DBH and proenkephalin A were more variable. Though the actual level of each mRNA was not determined, the identical value of DBH/proenkephalin A mRNAs ratio in the three tumors could suggest a coordination in the expression of these two proteins.