C-sequence of the Dictyostelium cytoplasmic dynein participates in processivity modulation.
FEBS Lett
; 585(8): 1185-90, 2011 Apr 20.
Article
em En
| MEDLINE
| ID: mdl-21420957
ABSTRACT
We examined the functional roles of C-sequence, a 47-kDa non-AAA+ module at the C-terminal end of the 380-kDa Dictyostelium dynein motor domain. When the distal segment of the C-sequence was deleted from the motor domain, the single-molecule processivity of the dimerized motor domain was selectively impaired without its ensemble motile ability and ATPase activity being severely affected. When the hinge-like sequence between the distal and proximal C-sequence segments was made more or less flexible, the dimeric motor showed lower or higher processivity, respectively. These results suggest a potential function of the distal C-sequence segment as a modulator of processivity.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Trifosfato de Adenosina
/
Dictyostelium
/
Dineínas do Citoplasma
/
Microtúbulos
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Japão