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Detection of herpes viral genomes in normal and diseased corneal epithelium.
Crouse, C A; Pflugfelder, S C; Pereira, I; Cleary, T; Rabinowitz, S; Atherton, S S.
Afiliação
  • Crouse CA; Bascom Palmer Eye Institute, Department of Ophthalmology, Miami, FL.
Curr Eye Res ; 9(6): 569-81, 1990 Jun.
Article em En | MEDLINE | ID: mdl-2167192
Herpetic ocular disease is one of the major causes of corneal blindness. Clinical diagnosis of corneal disease is based principally on corneal appearance. However, abnormal morphology of the corneal epithelium (CE) is not an indicator for the presence of a herpes virus. Further, it has not been established if herpes viruses are present in normal corneal epithelial tissue. In these studies, the polymerase chain reaction was used to evaluate normal and diseased corneal epithelium for the presence of herpes simplex virus type 1 (HSV-1), Epstein-Barr virus (EBV) and cytomegalovirus (CMV) genomic sequences. Thirty-two normal corneal epithelium specimens obtained from cadavers shortly after death were analyzed for HSV-1, EBV and CMV genomic sequences. Three of the 32 normal CE specimens were positive for amplified EBV DNA, 1 was positive for HSV-1 DNA, and none was positive for CMV DNA. We also tested eight herpetic dendritic lesions of which 3 were HSV-1 culture and PCR positive. The remaining five dendritic lesions were HSV-1 culture and PCR negative. Since these lesions were not evaluated for other herpesviruses, the etiology of these dendritic lesions is unknown. Six corneal epithelium samples from HIV-infected donors were negative for EBV, CMV and HSV-1 amplified sequences. Positive EBV, CMV and HSV-1 serology on all normal donors and on donors with clinically apparent disease did not correlate with positive PCR results. The results of these studies suggest that EBV and HSV-1 DNA can be amplified from a small percentage of apparently normal corneal epithelium.
Assuntos
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Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: DNA Viral / Amplificação de Genes / Ceratite Dendrítica / Reação em Cadeia da Polimerase / Simplexvirus / Córnea Tipo de estudo: Diagnostic_studies Limite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Curr Eye Res Ano de publicação: 1990 Tipo de documento: Article
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Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: DNA Viral / Amplificação de Genes / Ceratite Dendrítica / Reação em Cadeia da Polimerase / Simplexvirus / Córnea Tipo de estudo: Diagnostic_studies Limite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Curr Eye Res Ano de publicação: 1990 Tipo de documento: Article