Single-step conjugation of antibodies to quantum dots for labeling cell surface receptors in mammalian cells.
Methods Mol Biol
; 751: 553-63, 2011.
Article
em En
| MEDLINE
| ID: mdl-21674354
ABSTRACT
Labeling of cell surface receptors in living cells can be achieved using antibody-conjugated semiconductor quantum dots (QDs). The inherent photostable property of QDs can be exploited for understanding the arrangement and distribution of receptors in the plasma membrane. We describe herein methods that allow conjugation of antibodies to QDs in a single step without the formation of side products. This protocol can be adapted universally for any type of QD structure with a coating of free amino groups.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
/
Pontos Quânticos
/
Receptores ErbB
/
Anticorpos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2011
Tipo de documento:
Article