Detecting apoptosis using dynamic light scattering with optical coherence tomography.
J Biomed Opt
; 16(7): 070505, 2011 Jul.
Article
em En
| MEDLINE
| ID: mdl-21806246
ABSTRACT
A dynamic light scattering technique is implemented using optical coherence tomography (OCT) to measure the change in intracellular motion as cells undergo apoptosis. Acute myeloid leukemia cells were treated with cisplatin and imaged at a frame rate of 166 Hz using a 1300 nm swept-source OCT system at various times over a period of 48 h. Time correlation analysis of the speckle intensities indicated a significant increase in intracellular motion 24 h after treatment. This rise in intracellular motion correlated with histological findings of irregularly shaped and fragmented cells indicative of cell membrane blebbing and fragmentation.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Tomografia de Coerência Óptica
Limite:
Humans
Idioma:
En
Revista:
J Biomed Opt
Assunto da revista:
ENGENHARIA BIOMEDICA
/
OFTALMOLOGIA
Ano de publicação:
2011
Tipo de documento:
Article