Dietary folate, but not choline, modifies neural tube defect risk in Shmt1 knockout mice.
Am J Clin Nutr
; 95(1): 109-14, 2012 Jan.
Article
em En
| MEDLINE
| ID: mdl-22134951
ABSTRACT
BACKGROUND:
Low dietary choline intake has been proposed to increase the risk of neural tube defects (NTDs) in human populations. Mice with reduced Shmt1 expression exhibit a higher frequency of NTDs when placed on a folate- and choline-deficient diet and may represent a model of human NTDs. The individual contribution of dietary folate and choline deficiency to NTD incidence in this mouse model is not known.OBJECTIVE:
To dissociate the effects of dietary folate and choline deficiency on Shmt1-related NTD sensitivity, we determined NTD incidence in embryos from Shmt1-null dams fed diets deficient in either folate or choline.DESIGN:
Shmt1(+/+) and Shmt1(-/-) dams were maintained on a standard AIN93G diet (Dyets), an AIN93G diet lacking folate (FD), or an AIN93G diet lacking choline (CD). Virgin Shmt1(+/+) and Shmt1(-/-) dams were crossed with Shmt1(+/-) males, and embryos were examined for the presence of NTDs at embryonic day (E) 11.5 or E12.5.RESULTS:
Exencephaly was observed only in Shmt1(-/-) embryos isolated from dams maintained on the FD diet (P = 0.004). Approximately 33% of Shmt1(-/-)embryos (n = 18) isolated from dams maintained on the FD diet exhibited exencephaly. NTDs were not observed in any embryos isolated from dams maintained on the CD (n = 100) or control (n = 152) diets or in any Shmt1(+/+) (n = 78) or Shmt1(+/-) embryos (n = 182).CONCLUSION:
Maternal folate deficiency alone is sufficient to induce NTDs in response to embryonic Shmt1 disruption.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Glicina Hidroximetiltransferase
/
Colina
/
Deficiência de Colina
/
Dieta
/
Ácido Fólico
/
Deficiência de Ácido Fólico
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Defeitos do Tubo Neural
Tipo de estudo:
Etiology_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Am J Clin Nutr
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos