Surfactant Protein D modulates allergen particle uptake and inflammatory response in a human epithelial airway model.
Respir Res
; 13: 8, 2012 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-22296755
ABSTRACT
BACKGROUND:
Allergen-containing subpollen particles (SPP) are released from whole plant pollen upon contact with water or even high humidity. Because of their size SPP can preferentially reach the lower airways where they come into contact with surfactant protein (SP)-D. The aim of the present study was to investigate the influence of SP-D in a complex three-dimensional human epithelial airway model, which simulates the most important barrier functions of the epithelial airway. The uptake of SPP as well as the secretion of pro-inflammatory cytokines was investigated.METHODS:
SPP were isolated from timothy grass and subsequently fluorescently labeled. A human epithelial airway model was built by using human Type II-pneumocyte like cells (A549 cells), human monocyte derived macrophages as well as human monocyte derived dendritic cells. The epithelial cell model was incubated with SPP in the presence and absence of surfactant protein D. Particle uptake was evaluated by confocal microscopy and advanced computer-controlled analysis. Finally, human primary CD4+ T-Cells were added to the epithelial airway model and soluble mediators were measured by enzyme linked immunosorbent assay or bead array.RESULTS:
SPP were taken up by epithelial cells, macrophages, and dendritic cells. This uptake coincided with secretion of pro-inflammatory cytokines and chemokines. SP-D modulated the uptake of SPP in a cell type specific way (e.g. increased number of macrophages and epithelial cells, which participated in allergen particle uptake) and led to a decreased secretion of pro-inflammatory cytokines.CONCLUSION:
These results display a possible mechanism of how SP-D can modulate the inflammatory response to inhaled allergen.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Asma
/
Proteína D Associada a Surfactante Pulmonar
/
Células Epiteliais Alveolares
/
Inflamação
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Respir Res
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Alemanha