Quantitative image analysis of gamma-H2AX foci induced by ionizing radiation applying open source programs.
Anal Quant Cytol Histol
; 34(2): 66-71, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22611761
ABSTRACT
OBJECTIVE:
To test a CellProfiler pipeline for automated counting and characterization of gamma-H2AX foci in color images of human cultured cells. STUDYDESIGN:
A431 cells were irradiated and stained for gamma-H2AX foci detection. Sets of color images were analyzed visually, and findings were compared with those using FociCounter and CellProfiler software.RESULTS:
The CellProfiler pipeline includes some proprieties not present in FociCounter, such as the automatic detection of nuclei, the detection of touching nuclei and the rejection of nuclei that touch the border of the image. The time required for manual operation is associated with the number of images analyzed visually or by FociCounter but not for the CellProfiler program. CellProfiler reduced manual operation time and is about 4 times faster than semiautomatic detection using FociCounter and 10 times faster than visual counting.CONCLUSION:
We conclude that CellProfiler and FociCounter are reliable tools for measuring gamma-H2AX foci. However, CellProfiler overcomes the limitations of the FociCounter program and is able to detect nuclei automatically, saving considerable manual operation.
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Coleções:
01-internacional
Temas:
Geral
/
Prevencao_e_fatores_de_risco
/
Agentes_cancerigenos
/
Radiacao_solar
/
Tipos_de_cancer
/
Pele
Base de dados:
MEDLINE
Assunto principal:
Patologia Clínica
/
Neoplasias Cutâneas
/
Dano ao DNA
/
Processamento de Imagem Assistida por Computador
/
Histonas
Limite:
Humans
Idioma:
En
Revista:
Anal Quant Cytol Histol
Assunto da revista:
HISTOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Cuba