[The modified restriction of amino acids of HVR1, HVR2, HVR5, HVR7 in human adenovirus serotype 3 hexon].
Bing Du Xue Bao
; 28(4): 372-81, 2012 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-22978161
ABSTRACT
The limitation of traditional Ad vectors result in wide application of capsid-incorporation of antigens into adenovirus capsid proteins, but usually it can't rescue virus successfully when we engineered the hypervariable regions (HVRs) of hexon in adenovirus serotype 3(Ad3) vector. So we deleted or retained some amino acids in HVR1, HVR2, HVR5, HVR7 predicted by bioinformatics, constructed recombinant Ad3 vector pBRAddeltaE3GFP-mHexon, and transfected it into AD293 cell to confirm the influence on the virus rescue. These data of amino acids that can be deleted or retained in the HVRs of Ad3 vector should provide operating foundation for antigen capsid-incorporation strategy in human adenovirus serotype 3, and also lay the groundwork for application of expressing foreign antigens in the hexon of human adenovirus serotype 3 as a platform of multivalent vaccine vectors.
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Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Adenovírus Humanos
/
Proteínas do Envelope Viral
/
Biologia Computacional
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Bing Du Xue Bao
Assunto da revista:
VIROLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China