Conjugated linoleic acids alleviate the immunosuppression of peripheral blood T lymphocytes in broiler chickens exposed to cyclosporin A.

The immunoregulatory actions of conjugated linoleic acids (CLA) of relevance immunosuppression were investigated. To test the hypothesis that CLA ameliorate immunosuppression, we developed the immunosuppressive model of peripheral blood T lymphocytes in broiler chickens induced by cyclosporin A. Peripheral blood T lymphocytes of broiler chickens were cultured with media containing various concentrations (25, 50, 100, and 200 µmol/L) of c9, t11-CLA and t10, c12-CLA to investigate the effects of CLA isomers on peripheral blood T lymphocyte proliferation, interleukin-2, the activity of phospholipase C, and protein kinase C production. Results suggested that CLA alleviated the immunosuppression of T lymphocytes in broiler chickens exposed to cyclosporin A through increasing of peripheral blood T lymphocyte proliferation and interleukin-2. The 2 CLA isomers enhanced T lymphocyte proliferation at low concentration and inhibited T lymphocyte proliferation at high concentration. In addition, the effect of c9, t11-CLA was better than that of t10, c12-CLA. At the cellular level, the effects of CLA on the alleviation of immunosuppression in T lymphocytes are mainly attributable to increasing the signaling molecules, such as phospholipase C and protein kinase C.