Lymphangiography of the mouse tail.
Cold Spring Harb Protoc
; 2012(11): 1177-8, 2012 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-23118364
ABSTRACT
The lymphatic system is an important route for metastasis of cancer cells. Lymphatic microvessels have been studied by adapting lymphangiography to the mouse tail. In this technique, a high-molecular-weight fluorescently labeled tracer is injected into the interstitial compartment of the tail tissue. The tracer migrates to the local lymphatic vessels and fills the network, allowing visualization. By implanting a tumor in the tail, the structure and function of the lymphatics at the tumor periphery can be monitored. This protocol describes a method for injecting tumor cells into a mouse tail and for microlymphangiography.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Cauda
/
Linfografia
Limite:
Animals
Idioma:
En
Revista:
Cold Spring Harb Protoc
Ano de publicação:
2012
Tipo de documento:
Article