Identification of an anticancer compound against HT-29 cells from Phellinus linteus grown on germinated brown rice.
Asian Pac J Trop Biomed
; 3(10): 785-9, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-24075343
ABSTRACT
OBJECTIVE:
To isolate and identify the anticancer compound against proliferation of human colon cancer cells from ethyl acetate (EtOAc) extract of Phellinus linteus grown on germinated brown rice (PB).METHODS:
EtOAc extract of PB was partitioned with n-hexane, EtOAc, and water-saturated n-butanol. Anticancer compound of n-hexane layer was isolated and identified by HPLC and NMR, respectively. Cytotoxicity against HT-29 cells was tested by SRB assay.RESULTS:
The n-hexane layer obtained after solvent fractionation of PB EtOAc extracts showed a potent anticancer activity against the HT-29 cell line. Atractylenolide I, a eudesmane-type sesquiterpene lactone, a major anticancer substance of PB, was isolated from the n-hexane layer by silica gel column chromatography and preparative-HPLC. This structure was elucidated by one- and two-dimensional NMR spectroscopic data. Atractylenolide I has not been reported in mushrooms or rice as of yet. The isolated compound dose-dependently inhibited the growth of HT-29 human colon cancer cells.CONCLUSIONS:
Atractylenolide I might contribute to the anticancer effect of PB.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Basidiomycota
/
Misturas Complexas
/
Antineoplásicos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Asian Pac J Trop Biomed
Ano de publicação:
2013
Tipo de documento:
Article