Drosophila UTX coordinates with p53 to regulate ku80 expression in response to DNA damage.
PLoS One
; 8(11): e78652, 2013.
Article
em En
| MEDLINE
| ID: mdl-24265704
ABSTRACT
UTX is known as a general factor that activates gene transcription during development. Here, we demonstrate an additional essential role of UTX in the DNA damage response, in which it upregulates the expression of ku80 in Drosophila, both in cultured cells and in third instar larvae. We further showed that UTX mediates the expression of ku80 by the demethylation of H3K27me3 at the ku80 promoter upon exposure to ionizing radiation (IR) in a p53-dependent manner. UTX interacts physically with p53, and both UTX and p53 are recruited to the ku80 promoter following IR exposure in an interdependent manner. In contrast, the loss of utx has little impact on the expression of ku70, mre11, hid and reaper, suggesting the specific regulation of ku80 expression by UTX. Thus, our findings further elucidate the molecular function of UTX.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases N-Desmetilantes
/
Dano ao DNA
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Regulação da Expressão Gênica
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Proteína Supressora de Tumor p53
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Proteínas de Drosophila
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Antígenos Nucleares
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Proteínas de Ligação a DNA
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Drosophila melanogaster
Limite:
Animals
Idioma:
En
Revista:
PLoS One
Assunto da revista:
CIENCIA
/
MEDICINA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China