Intrastromal application of riboflavin for corneal crosslinking.
Invest Ophthalmol Vis Sci
; 55(7): 4261-5, 2014 Jun 10.
Article
em En
| MEDLINE
| ID: mdl-24917136
ABSTRACT
PURPOSE:
To experimentally evaluate the efficacy of corneal crosslinking (CXL) by injecting the photomediator riboflavin into the corneal stroma via intrastromal channels.METHODS:
Five groups of pig corneas, nine each, were compared regarding stress-strain relationship and UV-absorption. Group 1 had intrastromal channels floated with riboflavin 0.5%-solution followed by UVA-irradiation (3 mW/cm(2) for 30 minutes); group 2 was handled like group 1, but were irradiated with 9 mW/cm(2) for 10 minutes; group 3 was treated according to the Dresden protocol (epi-off, 9 mW/cm(2) for 10 minutes); group 4 had the identical channel system, no riboflavin but identical irradiation; group 5 with native corneas served as a control group. The intrastromal channels were created with a femtosecond laser. The stress-strain relations were measured in corneal strips using a uniaxial material tester at strains up to 12%. The UV-transmission of the corneas was measured in groups 1, 3, and 5.RESULTS:
The stress needed for a 10% strain was significantly increased by 82% in the corneas treated with the Dresden protocol compared with native cornea (P = 0.0005). With intrastromal application of riboflavin the significant increase was 87% (P = 0.0005) in group 1 and 64% (P = 0.007) in group 2. The channel formation alone did not alter biomechanics (P = 0.923). The corneal UVA-transmission was 2.4% after intrastromal riboflavin application, 8.9% after the treatment according to the Dresden protocol, and 57.9% in native corneas.CONCLUSIONS:
The experiments demonstrate the intrastromal application of riboflavin by means of intrastromal channels a feasible "epi-on" approach for CXL. More experimental data are needed before clinical testing.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Fotoquimioterapia
/
Riboflavina
/
Colágeno
/
Substância Própria
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Reagentes de Ligações Cruzadas
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Ceratocone
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Revista:
Invest Ophthalmol Vis Sci
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Suíça