Analysis by high throughput sequencing of Specific Pathogen Free eggs.
Biologicals
; 42(4): 218-9, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24930452
ABSTRACT
Specific Pathogen Free (SPF) embryonated eggs are used for the production of many veterinary and human vaccines. We have used High Throughput Sequencing to screen allantoic fluids and embryos for the presence of encapsidated viral genomes and viral transcripts, respectively. SPF eggs from two different producers were tested. We evidenced sequences corresponding to known endogenous retroviruses and sequences of Avian Leukosis Virus, but no sequence that might suggest a productive infection of eggs with a virus even distant from known viruses. Our results strongly suggest that SPF eggs such as those used for this study represent a safe substrate for the production of vaccines.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Organismos Livres de Patógenos Específicos
/
Ovos
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Sequenciamento de Nucleotídeos em Larga Escala
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Biologicals
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
França