ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.
PLoS One
; 9(6): e100342, 2014.
Article
em En
| MEDLINE
| ID: mdl-24949947
ABSTRACT
OBJECTIVE:
To develop a new ImmunoFISH technique for the study of oligodendrogliomas by combining a standard immunohistochemical stain using MIB-1 antibody with a standard FISH technique using commercial 1p36 and 19q13 chromosomal probes.METHODS:
Validation was performed by two observers on a series of 36 pre-selected oligodendrogliomas and compared to the results previously determined by FISH alone.RESULTS:
The ImFISH technique is easy to perform and to analyze and is no more time-consuming than the usual FISH technique. Our results show that the inter-observer reliability of ImFISH is high (κâ=â0.86 and 0.95 respectively for 1p and 19q). Compared to FISH, the ImFISH exhibits a very high sensitivity (â¼100%) and specificity (â¼90%) for 1p and/or 19q deleted cases. The sensitivity is high for normal cases (â¼85%) and imbalanced cases (â¼90%) with a specificity ranging between 50 and 85%. Finally, there were no significant differences between FISH and ImFISH results calculated on 60, 40 or 20 cells.CONCLUSION:
Our study demonstrates the reliability of the ImFISH technique in oligodendrogliomas and emphasizes its advantage in poorly cellular tumoral specimen.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
/
Tipos_de_cancer
/
Outros_tipos
Base de dados:
MEDLINE
Assunto principal:
Oligodendroglioma
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Cromossomos Humanos Par 1
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Cromossomos Humanos Par 19
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Neoplasias Encefálicas
/
Anticorpos Antinucleares
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Hibridização in Situ Fluorescente
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Anticorpos Monoclonais
Limite:
Adult
/
Aged
/
Aged80
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Female
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Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
PLoS One
Assunto da revista:
CIENCIA
/
MEDICINA
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Canadá