Characterization of a biopharmaceutical protein and evaluation of its purification process using automated capillary Western blot.
Electrophoresis
; 36(2): 363-70, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25262923
ABSTRACT
This paper describes the application of an automated size-based capillary Western blot system (Sally instrument) from ProteinSimple, Inc., for biopharmaceutical fusion-Fc protein characterization and evaluation of its purification process. The fusion-Fc protein column purification from an excess of single chain Fc polypeptide and removal of an enzyme coexpressed for protein maturation have been demonstrated using an automated capillary Western system. The clearance of a selected host cell protein (HCP) present in cell culture of fusion-Fc protein was also quantitatively monitored throughout the protein purification process. Additionally, the low levels of fusion-Fc product-related impurities detected by traditional slab gel Western blot were confirmed by the automated capillary Western system. Compared to the manual approach, the automated capillary Western blot provides the advantages of ease of operation, higher sample throughput, greater linearity range, and higher precision for protein quantitation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Western Blotting
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Eletroforese Capilar
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos