Tip-enhanced laser ablation sample transfer for biomolecule mass spectrometry.
J Am Soc Mass Spectrom
; 26(1): 63-70, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25287125
ABSTRACT
Atomic force microscope (AFM) tip-enhanced laser ablation was used to transfer molecules from thin films to a suspended silver wire for off-line mass spectrometry using laser desorption ionization (LDI) and matrix-assisted laser desorption ionization (MALDI). An AFM with a 30 nm radius gold-coated silicon tip was used to image the sample and to hold the tip 15 nm from the surface for material removal using a 355 nm NdYAG laser. The ablated material was captured on a silver wire that was held 300 µm vertically and 100 µm horizontally from the tip. For the small molecules anthracene and rhodamine 6G, the wire was cut and affixed to a metal target using double-sided conductive tape and analyzed by LDI using a commercial laser desorption time-of-flight mass spectrometer. Approximately 100 fg of material was ablated from each of the 1 µm ablation spots and transferred with approximately 3% efficiency. For larger polypeptide molecules angiotensin II and bovine insulin, the captured material was dissolved in saturated matrix solution and deposited on a target for MALDI analysis.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Lasers
Limite:
Animals
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos