Your browser doesn't support javascript.
loading
New serine-derived gemini surfactants as gene delivery systems.
Cardoso, Ana M; Morais, Catarina M; Cruz, A Rita; Silva, Sandra G; do Vale, M Luísa; Marques, Eduardo F; de Lima, Maria C Pedroso; Jurado, Amália S.
Afiliação
  • Cardoso AM; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal; Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
  • Morais CM; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal; Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
  • Cruz AR; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal; Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.
  • Silva SG; Centro de Investigação em Química, Department of Chemistry and Biochemistry, University of Porto, Porto, Portugal.
  • do Vale ML; Centro de Investigação em Química, Department of Chemistry and Biochemistry, University of Porto, Porto, Portugal.
  • Marques EF; Centro de Investigação em Química, Department of Chemistry and Biochemistry, University of Porto, Porto, Portugal.
  • de Lima MC; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal; Department of Life Sciences, University of Coimbra, Coimbra, Portugal.
  • Jurado AS; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal; Department of Life Sciences, University of Coimbra, Coimbra, Portugal. Electronic address: asjurado@bioq.uc.pt.
Eur J Pharm Biopharm ; 89: 347-56, 2015 Jan.
Article em En | MEDLINE | ID: mdl-25513958
Gemini surfactants have been extensively used for in vitro gene delivery. Amino acid-derived gemini surfactants combine the special aggregation properties characteristic of the gemini surfactants with high biocompatibility and biodegradability. In this work, novel serine-derived gemini surfactants, differing in alkyl chain lengths and in the linker group bridging the spacer to the headgroups (amine, amide and ester), were evaluated for their ability to mediate gene delivery either per se or in combination with helper lipids. Gemini surfactant-based DNA complexes were characterized in terms of hydrodynamic diameter, surface charge, stability in aqueous buffer and ability to protect DNA. Efficient formulations, able to transfect up to 50% of the cells without causing toxicity, were found at very low surfactant/DNA charge ratios (1/1-2/1). The most efficient complexes presented sizes suitable for intravenous administration and negative surface charge, a feature known to preclude potentially adverse interactions with serum components. This work brings forward a new family of gemini surfactants with great potential as gene delivery systems.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Serina / Tensoativos / DNA / Compostos de Amônio Quaternário Limite: Humans Idioma: En Revista: Eur J Pharm Biopharm Assunto da revista: FARMACIA / FARMACOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Portugal

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Serina / Tensoativos / DNA / Compostos de Amônio Quaternário Limite: Humans Idioma: En Revista: Eur J Pharm Biopharm Assunto da revista: FARMACIA / FARMACOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Portugal