Secretome analysis of human articular chondrocytes unravels catabolic effects of nicotine on the joint.
Proteomics Clin Appl
; 10(6): 671-80, 2016 06.
Article
em En
| MEDLINE
| ID: mdl-25914226
ABSTRACT
PURPOSE:
Osteoarthritis (OA) is a degenerative joint pathology characterized by articular cartilage degradation that lacks from efficient therapy. Since previous epidemiological data show a high controversy regarding the role of smoking in OA, we aimed to evaluate the effects of nicotine (the most physiologically active compound of tobacco) on the joint. EXPERIMENTALDESIGN:
Secretome analyses, based on metabolic labeling followed by LC-MALDI-TOF/TOF analysis, were carried out using an in vitro model of articular inflammation (primary human articular chondrocytes treated with interleukin-1ß), and also on osteoarthritic cells. ELISA and Western blot assays were performed to verify some of the results.RESULTS:
Nineteen proteins were altered by nicotine in the model of articular inflammation, including several cytokines and proteases. We confirmed the increased secretion by nicotine of matrix metalloproteinase 1 and two proposed markers of OA, fibronectin, and chitinase 3-like protein 1. Finally, four components of the extracellular matrix of cartilage were decreased by nicotine in OA chondrocytes. CONCLUSIONS AND CLINICAL RELEVANCE Our data contribute to a better understanding of the molecular mechanisms that are modulated by nicotine in cartilage cells, suggesting a negative effect of this drug on the joint.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Osteoartrite
/
Fibronectinas
/
Condrócitos
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Metaloproteinase 1 da Matriz
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Proteína 1 Semelhante à Quitinase-3
/
Metabolismo
/
Nicotina
Tipo de estudo:
Observational_studies
Limite:
Aged
/
Aged80
/
Female
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Humans
/
Male
Idioma:
En
Revista:
Proteomics Clin Appl
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Espanha