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Estradiol via estrogen receptor beta inhibits chondrogenesis of mouse vertebral growth plate in vitro.
Zeng, Kefeng; Zhang, Hong-Qi; Chen, Yong; Gao, Qile.
Afiliação
  • Zeng K; Department of Spine Surgery, Xiangya Hospital of Central South University, Xiangya Road 87, Changsha, 410008, China.
  • Zhang HQ; Department of Spine Surgery, Xiangya Hospital of Central South University, Xiangya Road 87, Changsha, 410008, China. zhq9996@163.com.
  • Chen Y; Department of Spine Surgery, Xiangya Hospital of Central South University, Xiangya Road 87, Changsha, 410008, China.
  • Gao Q; Department of Spine Surgery, Xiangya Hospital of Central South University, Xiangya Road 87, Changsha, 410008, China.
Childs Nerv Syst ; 32(3): 461-5, 2016 Mar.
Article em En | MEDLINE | ID: mdl-26631067
PURPOSE: Abnormal growth of vertebral growth plate (VGP) was considered as one of the etiologic factors in adolescent idiopathic scoliosis (AIS). Previous studies described that estrogen played an important role in the pathogenesis of AIS. The present study was aimed to investigate the effect of estrogen/estrogen receptor axis on mouse VGP chondrocytes in vitro. METHODS: Chondrocytes were isolated from mouse VGP and treated with or without 17ß-estradiol (E2). Cell proliferation was measured by the cell growth rate assay. Gene expression of collagen type II and aggrecan were evaluated by real-time PCR. Expression of the proliferating cell nuclear antigen (PCNA), Sox9, and Smad4 were detected by Western blotting. RESULTS: Estradiol inhibited the proliferation of VGP chondrocytes and the gene expression of collagen type II and aggrecan and downregulated the protein expression of PCNA, Sox9, and Smad4. In addition, the inhibitory effect of estradiol was reversed by ERß small interfering RNA (siRNA) or PHTPP, an ERß antagonist. CONCLUSIONS: Estradiol via estrogen/estrogen receptor ß axis inhibits the proliferation and differentiation of VGP chondrocytes, which might give some new insight into the regulatory mechanism of bone development.
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Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Condrogênese / Receptor beta de Estrogênio / Estradiol / Lâmina de Crescimento Limite: Animals Idioma: En Revista: Childs Nerv Syst Assunto da revista: NEUROLOGIA / PEDIATRIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Condrogênese / Receptor beta de Estrogênio / Estradiol / Lâmina de Crescimento Limite: Animals Idioma: En Revista: Childs Nerv Syst Assunto da revista: NEUROLOGIA / PEDIATRIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China