Tumor suppressive miR-196a is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma.
Mol Med Rep
; 14(1): 560-6, 2016 Jul.
Article
em En
| MEDLINE
| ID: mdl-27175581
ABSTRACT
Certain microRNAs (miRs) are implicated in the genesis and progression of various cancers by regulating multiple cellular processes, including apoptosis, proliferation and migration. The aim of the present study was to explore the functions of miR196a in renal cell carcinoma (RCC). RCC and paired normal tissues we assessed for miR196a expression by reverse-transcription quantitative PCR. Furthermore, the effects of miR196a on renal cell proliferation, apoptosis and migration were determined using an MTT assay, flow cytometry and a scratch wound assay following restoration of miR-196a with synthetic mimics. miR196a was found to be significantly downregulated in RCC tissues compared with that in normal tissues (P<0.05). In addition, miR196a suppressed cell proliferation, apoptosis and migration of the 786O and ACHN RCC cell lines. To the best of our knowledge, the present study was the first to report this tumor suppressor role of miR196a in RCC. The results indicated that miR196a may be a potential diagnostic biomarker for RCC and that transfection of miR-196a mimics may represent a novel treatment strategy for RCC.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
/
Tipos_de_cancer
/
Outros_tipos
Base de dados:
MEDLINE
Assunto principal:
Carcinoma de Células Renais
/
Genes Supressores de Tumor
/
MicroRNAs
/
Neoplasias Renais
Tipo de estudo:
Risk_factors_studies
Limite:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2016
Tipo de documento:
Article