Critical roles of adenosine A2A receptor in regulating the balance of Treg/Th17 cells in allergic asthma.
Clin Respir J
; 12(1): 149-157, 2018 Jan.
Article
em En
| MEDLINE
| ID: mdl-27216911
ABSTRACT
INTRODUCTION:
Deficiency of Treg cells and hyperactivity of Th17 cells together are involved in the immunological pathogenesis of asthma. The adenosine A2A receptor (A2AR) plays a critical role in the increased Foxp3 expression of Treg cells and the decreased Th17 generation.OBJECTIVE:
The study aimed to investigate A2AR expression in peripheral blood and its regulatory effect on balance of Treg/Th17 cells in asthma.METHODS:
Thirty-one patients with chronic persistent asthma were recruited and divided into 18 intermittent to mild asthma patients, 13 moderate to severe asthma patients. A2AR, Foxp3, and ROR-γt mRNA expression levels in peripheral blood mononuclear cells (PBMCs) were measured by quantitative polymerase chain reaction (qPCR). TGF-ß, IL-17, and IgE in plasma were detected with enzyme-linked immunosorbent assay (ELISA). Forty-two BALB/c mice were randomly, equally assigned to control group, ovalbumin (OVA) group and OVA + CGS (CGS21680, A2AR agonist) group. The infiltration of lung inflammation cells were evaluated by HE, A2AR, Foxp3, and ROR-γt mRNA in lung tissues measured by qPCR, TGF-ß, IL-17, and IgE in plasma measured with ELISA, and IL-17 and TGF-ß protein in lung tissues analyzed with immunohistochemical.RESULTS:
Our results showed that expression A2AR mRNA in PBMCs was associated with asthma severity. Foxp3 mRNA, TGF-ß, and FEV1%pred positively correlated with A2AR mRNA in asthma. ROR-γt mRNA and IL-17 negatively correlated with A2AR mRNA in asthma. CGS could promote Foxp3 mRNA expression, TGF-ß, and improve lung function while inhibit ROR-γt mRNA expression, IL-17, and the infiltration of lung inflammation cells.CONCLUSION:
A2AR could regulate the balance of Treg/Th17 cells in asthma.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Asma
/
RNA Mensageiro
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Regulação da Expressão Gênica
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Linfócitos T Reguladores
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Receptor A2A de Adenosina
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Células Th17
Limite:
Adult
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Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Clin Respir J
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China